|
KMID : 0613820110210010089
|
|
Journal of Life Science
2011 Volume.21 No. 1 p.89 ~ p.95
|
|
|
Apoptotic Effects of Curcumin and EGCG via Akt-p53 Signaling Pathway in HCT116 Colon Cancer Cells
|
|
Park Song-Yi
Lee Sol-Hwa
Park Ock-Jin
Kim Young-Min
|
|
|
Abstract
|
|
|
|
p53 is tumor suppressor gene that regulates apoptosis such as caspase-dependent and p21-mediated signaling pathways. PI3K/Akt is known to be over-activated in cancer cells. Akt activates many survival-related signals such as mTOR and COX-2. Inactivation of Akt would result in non-inhibition of p53 as well as induced apoptosis. In this study, we showed that curcumin and EGCG activate p53 via inhibition of the Akt signaling pathway. Treatments using curcumin and EGCG in different concentrations for 24 hr and 48 hr inhibited proliferation of HCT116 colon cancer cells and increased apoptotic cell death. Also, our data showed that curcumin and EGCG increased the p53 expression and decreased the p-Akt. Treatment of LY294002 (Akt inhibitor) resulted in decreased cell proliferation of cancer cells, while LY294002 treated with curcumin or EGCG showed a greater decrease of cell proliferation. In addition, inhibition of Akt induced p53 activation in HCT116 colon cancer cells. These results suggest that curcumin and EGCG induce apoptosis by inhibiting Akt and increase p53 in HCT116 colon cancer cells.
|
|
|
KEYWORD
|
|
|
Curcumin, EGCG, apoptosis, HCT116 colon cancer cells, p53, Akt
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|